Study of Oxygen Consumption to Measure Energy Metabolism...

Study of Oxygen Consumption to Measure Energy Metabolism in Mammals Brianne Simonsen Lab Section 8 4 Oct. 2005 Abstract In this experiment the oxygen consumption of a mouse in environments of varying temperatures was studied. The hypothesis behind this experiment was that more oxygen would be consumed at a lower temperature to maintain body heat. To measure the rate of oxygen consumption a mouse was given a specific amount of oxygen. The amount consumed in a specific time was measured and compared to the rates at a colder temperature. Just as expected, oxygen was consumed more rapidly at a lower temperature. This would lead to the conclusion that more oxygen is required to maintain homeostasis in a system. Introduction†¦show more content†¦Before the experiment could begin several preparatory steps had to be taken. The respirometers had to be charged by placing a test tube of KOH pellets into the jar. The apparatus had to then be tested before the mouse could be placed inside and the data collection portion of the experiment commenced. The jar was dried and a piece of dry paper towel was placed in the bottom. The pipette end of the tubing was placed into a beaker of ink. The pinch clamp on the other end of the tubing was opened completely and the pipetor (blue handled) was attached to the end. The handle of the pipetor was pulled until ink on the other end of the tubing reached the bent portion of the pipette. The pinch clamp was pinched closed. The ink was studied and when it maintained a constant level after thirty seconds the preliminary testing went into its final stages. The sensitivity of the apparatus was then tested by warming the jar with body heat from the hands. The ink went down showing that the apparatus was correctly prepared. The apparatus was then taken apart. A mouse was weighed and placed inside the jar by the tail with the KOH pellets remaining in the test tube inside the apparatus. The respirometer was reassembled and a table was prepared to compare the movement of ink to the passage of time during the experiment. The respirometer was sealed and the ink was allowed to rise toShow MoreRelatedThe Effects Of Fitness Training On Vo2 Max And Vco2 Max1984 Words   |  8 PagesEffects of Fitness Training on VO2 max and VCO2 Max Abstract: Mammals consume oxygen and produce carbon dioxide as part of their metabolism process in order to maintain body functions. This occurs at all times, both rest and during intense work, within mammals. During times of greater work, skeletal muscles work harder than they do at rest. This in turn increases their metabolic rate and results in a greater demand for oxygen. It has been proposed that fitness training has a positive effectRead MoreSexually Transmitted Diseases35655 Words   |  143 Pageslife 72-76 Unit 4 Energy in the Community Electricity 77-78 Heat 78-81 Light 82-91 Sound 92 Simple Machines 93-99 Unit 5 The Physical Environment Weather and Climate 100-113 Soils 114-128 Unit 6 Living things in the Environment Plant and animal cells 129 Eukaryotes vs prokaryotes 130 Structures and functions 133-137 Microbes 148-150 Onion and cheek cells 150-151 Ecological study 154 Seed structureRead MoreHemp Cultivation in China42289 Words   |  170 PagesEstimates based on local data collected by interviewing village leaders and agricultural advisors, and the trends extrapolated from these data, lead to the conclusion that closer to 4,000 ha of land was used to grow hemp in 1993. The remainder of this study concerns recent hemp production in Tai an District along the Da Wen river south and west of Tai an city. This area includes Dong Ping County (south and east of Dong Ping Lake), Tai an Urban District (near the villages of Da Wen Kou and Ma Zhuang)